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Biodegradation of 5-chloro-2-picolinic acid by novel identified co-metabolizing degrader Achromobacter sp f1
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作者:Wu, ZG (Wu, Zhi-Guo); Wang, F (Wang, Fang) ; Ning, LQ (Ning, Li-Qun) ; Stedtfeld, RD (Stedtfeld, Robert D.); Yang, ZZ (Yang, Zong-Zheng) ; Cao, JG (Cao, Jing-Guo); Sheng, HJ (Sheng, Hong-Jie) ; Jiang, X (Jiang, Xin) 

 

题目:Biodegradation of 5-chloro-2-picolinic acid by novel identified co-metabolizing degrader Achromobacter sp f1

 

刊物:BIODEGRADATION,卷: 28  期: 2-3  页: 139-144

DOI: 10.1007/s10532-017-9783-z

出版年: MAY 2017

 

文章下载:http://download.springer.com/static/pdf/786/art%253A10.1007%252Fs10532-017-9783-z.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs10532-017-9783-z&token2=exp=1497408194~acl=%2Fstatic%2Fpdf%2F786%2Fart%25253A10.1007%25252Fs10532-017-9783-z.pdf%3ForiginUrl%3Dhttp%253A%252F%252Flink.springer.com%252Farticle%252F10.1007%252Fs10532-017-9783-z*~hmac=17f5c61d7f28ffe86ba4b7b733dc4d3eebad02990d5072eea61cd59dedc5ae01

 

摘要: 

Several bacteria have been isolated to degrade 4-chloronitrobenzene. Degradation of 4-chloronitrobenzene by Cupriavidus sp. D4 produces 5-chloro-2-picolinic acid as a dead-end by-product, a potential pollutant. To date, no bacterium that degrades 5-chloro-2-picolinic acid has been reported. Strain f1, isolated from a soil polluted by 4-chloronitrobenzene, was able to co-metabolize 5-chloro-2-picolinic acid in the presence of ethanol or other appropriate carbon sources. The strain was identified as Achromobacter sp. based on its physiological, biochemical characteristics, and 16S rRNA gene sequence analysis. The organism completely degraded 50, 100 and 200 mg L-1 of 5-chloro-2-picolinic acid within 48, 60, and 72 h, respectively. During the degradation of 5-chloro-2-picolinic acid, Cl- was released. The initial metabolic product of 5-chloro-2-picolinic acid was identified as 6-hydroxy-5-chloro-2-picolinic acid by LC-MS and NMR. Using a mixed culture of Achromobacter sp. f1 and Cupriavidus sp. D4 for degradation of 4-chloronitrobenzen, 5-chloro-2-picolinic acid did not accumulate. Results infer that Achromobacter sp. f1 can be used for complete biodegradation of 4-chloronitrobenzene in remedial applications.

 

 

 

 

 

 

 

 

 

 


 

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